Process of purifying recombinant proteins and compounds useful in such process
US5594115A · kind A · utility
40Cited by
4References
15Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Dec 29, 1994 |
| Grant date | Jan 14, 1997 |
| Priority date | — |
| Expiry date | Dec 29, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2740/16222
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Recombinant fusion proteins which comprise a biologically active polypeptide portion and metal chelating peptide are disclosed. The fusion proteins contain metal chelating peptides which have at least three and preferably six alternating histidine residues. Fusion proteins which contain metal chelating peptides that are substrates for dipeptidylpeptidase I are also disclosed. Additionally, a method of obtaining desired polypeptides by producing recombinant fusion proteins and purifying them with immobilized metal ions is disclosed. A kit for purifying desired polypeptides is also disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.