Detection of biopolymers and biooligomers with boron hydride labels
US5595878A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 2, 1995 |
| Grant date | Jan 21, 1997 |
| Priority date | — |
| Expiry date | Jun 2, 2015 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/68
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method is disclosed for detecting boron labeled biopolymers, biooligomers, and other species that bind to the biopolymers, and biooligomers with specificity. The biopolymers include RNA, DNA and proteins. The biooligomers include RNA oligomers, DNA oligomers, and peptides. The biopolymers and biooligomers are labeled with a borane, or a boron-hydride moiety. The borane and boron-hydride moiety are employed to reduce a metal ion, e.g. silver, to metal or metal oxide, metal boride or other metal species, or alternatively to reduce a dye, or another organic or inorganic compound. The product of the reduction reaction can be visualized with or without signal augmentation, by one or more means, including the naked eye or a microscope, or can be detected through the use of analytical equipment that can detect conductivity, voltage, density, ultraviolet or visible light, fluorescence, luminescence, phosphorescence, paramagnetism, magnetic susceptibility, or any other detectable physical property. The invention provides methods for selectively detecting biopolymers and biooligomers that contain such a boron-label, thereby permitting their selective identification within an environment wh…
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