Process for integration of a chosen gene on the chromosome of a bacterium using Mu transposons
US5595889A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 17, 1994 |
| Grant date | Jan 21, 1997 |
| Priority date | — |
| Expiry date | Nov 17, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/849
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a process for integration of a chosen gene or of a specific DNA molecule into the chromosome or episome of a bacterium by cloning the gene or DNA molecule into a defective transposon which is then integrated into the chromosomal or episomal DNA of the bacteria. The defective transposon is incapable of transposition autonomously but can be induced to transpose when properly complemented. The complementation to induce transposition can be limited so that the defective transposon produces a specific number of copies and thereafter is stable. The number of copies of the defective transposon produced during the period of transposition can be estimated by the level of expression of a marker gene contained within the defective transposon.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.