Interspersed repetitive element-bubble amplification of nucleic acids
US5597694A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 7, 1993 |
| Grant date | Jan 28, 1997 |
| Priority date | — |
| Expiry date | Oct 7, 2013 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/81
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for amplifying a nucleic acid fragment is described. A nucleic acid having interspersed repetitive elements is cleaved and the resulting nucleic acid fragments are ligated to a bubble oligonucleotide having two double stranded portions flanking a non-complementary portion consisting of two single strands to make bubble/nucleic acid units. These units are treated with a first primer that is complementary to at least a portion of the interspersed repetitive element, and with a second primer that is complementary to at least a portion of the extension product of the first primer, that portion containing sequences complementary to one of the single strands of the bubble oligonucleotide, under conditions which produce extension products of the primers. Also described are methods for labeling the products of the amplification, methods for detecting chromosomal aberrations and other uses of the amplification products.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.