In vitro amplification/expansion of CD34.sup.+ stem and progenitor cells
US5599703A · kind A · utility
44Cited by
4References
9Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 28, 1993 |
| Grant date | Feb 4, 1997 |
| Priority date | — |
| Expiry date | Oct 28, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2502/28
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention relates to a method of amplifying in vitro stemcells. In this method hematopoietic CD34.sup.+ stem and progenitor cells are isolated from human bone marrow and contacted with endothelial cells. The contacted stem cells and endothelial cells are cultured in the presence of at least one cytokine in an amount sufficient to support amplification/expansion of the hematopoietic CD34.sup.+ stem and progenitor cells. This method produces increased yields of hematopoietic CD34.sup.+ stem and progenitor cells which can be used in human therapeutics.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.