Patent · US Expired

Recombinant DNA encoding neuronal .alpha.-bungarotoxin-binding proteins

US5599709A · kind A · utility

3Cited by
0References
22Claims
0Family size

Assignee

Inventors

Key dates

Filing dateSep 28, 1989
Grant dateFeb 4, 1997
Priority date—
Expiry dateSep 28, 2009

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC07K14/705
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

An isolated DNA molecule encoding an .alpha.-bungarotoxin-binding protein (.alpha.BgtBP) subtype or a fragment of such .alpha. subunit is provided. The fragment of the .alpha. subunit is sufficiently homologous to specified DNA (FIG. 2A or FIG. 2B) so as to hybridize thereto under conditions of low stringency. Identification, characterization, isolation and sequencing of cDNA clones which encode two neuronal .alpha.BgtBP subunits, .alpha.1 and .alpha.2, is made possible. Such clones may be used as probes to identify further neuronal .alpha.BgtBP subunits, or as diagnostic tools to screen cholinergic agents and other drugs that may affect ligand binding, ion channel or other activity of intact neuronal .alpha.BgtBP subtypes.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.