Process for reactivating purified membrane proteins

US5599909A · kind A · utility

Assignee

• Behringwerke Aktiengesellschaft · DE

Inventors

• Karl Fickenscher · Marburg, DE
• Norbert Zander · Marburg, DE

Key dates

• Filing date: Dec 21, 1993
• Grant date: Feb 4, 1997
• Expiry date: Dec 21, 2013

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K14/745
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Processes for reactivating membrane proteins are disclosed. The membrane proteins are reactivated by mixing membrane proteins with phospholipids, in the absence of detergents, at acidic pH, followed by neutralization by buffer addition, or by mixing membrane proteins with phospholipids, in the absence of detergents, at elevated temperature, followed by buffer addition