Oligonucleotide N3'-P5' phosphoramidates: hybridization and nuclease resistance properties
US5599922A · kind A · utility
147Cited by
2References
32Claims
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Key dates
| Filing date | Mar 18, 1994 |
| Grant date | Feb 4, 1997 |
| Priority date | — |
| Expiry date | Mar 18, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07H21/00
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
Modified oligonucleotide 3'-NHP(O)(O.sup.-)O-5' phosphoramidates were synthesized on a solid phase support. The phosphoramidate analogs were found to have significantly increased resistance toward phosphodiesterase digestion. Thermal dissociation experiments demonstrated that these compounds form more stable duplexes than phosphodiesters with complementary DNA and particularly RNA strands. Further, the phosphoramidate analogs can also form stable triplexes with double-stranded DNA target, where under similar conditions parent phosphodiester compounds failed to do so.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.