Dry chemistry cascade immunoassay and affinity assay
US5601991A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Feb 13, 1995 |
| Grant date | Feb 11, 1997 |
| Priority date | — |
| Expiry date | Feb 13, 2015 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/112499
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method is described for performing an affinity assay comprising contacting a sample to be assayed for the presence of an analyte with a dry reagent containing the analyte (hapten, antigen, antibody, receptor, or complementary polynucleotide) bound to a reaction cascade initiator, an antibody or other binding pair partner reactive with said analyte, and magnetic particles, to form an assay mixture in a reaction chamber, incubating the assay mixture, applying an oscillating or moving static magnetic field to the assay mixture, activating the reaction cascade initiator to initiate a reaction cascade, monitoring the response of the magnetic particles to the oscillating or moving static magnetic field to provide a time varying signal, and determining the analyte concentration of the sample by analysis of the time varying signal, as well as a kit for performing the assay and a diagnostic system for performing the assay.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.