Purified Thermococcus barossii DNA polymerase
US5602011A · kind A · utility
7Cited by
8References
24Claims
0Family size
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Inventors
Key dates
| Filing date | Jan 18, 1995 |
| Grant date | Feb 11, 1997 |
| Priority date | — |
| Expiry date | Jan 18, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/1252
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A preparation of a purified DNA polymerase substantially identical to native DNA polymerase isolated from Thermococcus barossii is disclosed. In a preferable form of the present invention, the DNA polymerase is approximately 68 kDa in size, retains at least 90% activity after 8 hours of incubation at 95.degree. C. and retains between 60% and 80% proof-reading ability when catalyzing a polymerization reaction with a primer with a 3 base pair mismatch.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.