Method of detecting variant nucleic acids

US5605794A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Stephan Rust · Munster, DE
• Harald Funke · Munster, DE
• Gerd Assmann · Munster, DE
• Christoph Kessler · Munich, DE
• Rudiger Rueger · Seeshaupt, DE

Key dates

• Filing date: Mar 3, 1994
• Grant date: Feb 25, 1997
• Expiry date: Mar 3, 2014

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6858
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Method of detecting nucleic acids whose nucleotide sequences differ from one another at a position X. The method uses a set of oligonucleotides where the nucleotides found at position X are complementary to a variant nucleic acids only at this position X. However, with respect to the position of another mismatch Y, the nucleotide sequences thereof differ from one another. The invention further addresses reagents for performing the methods as well as reagents and applications of the method.