Method for preventing amplification of nucleic acid contaminants in amplification mixtures using nuclease-receptor conjugates
US5605796A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 19, 1994 |
| Grant date | Feb 25, 1997 |
| Priority date | — |
| Expiry date | Jul 19, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6848
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods and kits are disclosed for preventing amplification of contaminating copies of nucleic acids during in amplification of a nucleic acid suspected of being present in a sample. Modified nucleotides that render copies of the nucleic acid bindable by a member of a specific binding pair, such as a receptor, which does not bind to the nucleic acid, are incorporated into copies of the nucleic acid that are produced during the amplification. The sample is combined with an enzyme conjugate, usually a receptor bound to a nuclease, under conditions wherein prior to the amplification the member of a specific binding pair binds to the copies and the enzyme degrades the copies but not the nucleic acid. The methods and kits have particular application to the determination of a nucleic acid analyte.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.