Expression control sequences of the P-selectin gene

US5605821A · kind A · utility

Assignee

• The Board of Regents of the University of Oklahoma · US

Inventors

• Rodger P. McEver · Oklahoma City, US
• Junliang Pan · Moraga, US

Key dates

• Filing date: Aug 20, 1993
• Grant date: Feb 25, 1997
• Expiry date: Aug 20, 2013

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2830/85
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

DNA molecules and methods for the regulated expression of a gene in endothelial cells or megakaryocytes, are described, wherein the 5' flanking region of the P-selectin gene, or portions thereof, is ligated to the 5' end of a gene. The DNA molecules are also used as probes for screening individuals with abnormal levels of expression of P-selectin, or for production of pharmaceutical compositions to inhibit inflammation by inhibition of expression of P-selectin. These DNA molecules can also be used to identify and isolate previously unknown proteins which are involved in regulation of gene expression.