24 kilodalton cytoplasmic protease activating DNA fragmentation in apoptosis
US5605826A · kind A · utility
8Cited by
3References
10Claims
0Family size
Assignee
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Key dates
| Filing date | Jun 10, 1994 |
| Grant date | Feb 25, 1997 |
| Priority date | — |
| Expiry date | Jun 10, 2014 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K38/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
An apoptosis-associated protease having a relative mass of 24 kilodaltons and a defined amino acid composition is disclosed, together with a method for its purification from a cytoplasmic extract of mammalian cells treated with an apoptosis-inducing agent, such as tumor necrosis factor-.alpha. or UV irradiation, that comprises affinity chromatography with the serine protease inhibitor DK120 followed by heparin-sepharose chromatography. The protease has activity against the elastase-like substrate MAAPV and is capable of inducing apoptosis in isolated U937 cell target nuclei.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.