Displacement chromatography of proteins using low molecular weight anionic displacers
US5606033A · kind A · utility
20Cited by
6References
17Claims
0Family size
Inventors
Key dates
| Filing date | Oct 19, 1995 |
| Grant date | Feb 25, 1997 |
| Priority date | — |
| Expiry date | Oct 19, 2015 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N30/02
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
A method for the purification of proteins by displacement chromatography on ion exchange media using low molecular weight displacers is disclosed. Several classes of low molecular weight anionic species are exemplified, including aromatic rings having sulfonic acid or carboxylic acid moieties attached thereon, sulfated sugar derivatives, anionic antibiotics, and dendrimeric polymers. Novel compounds useful as displacers are dendrimers of formula ##STR1## wherein R.sup.1 is lower alkyl, n is 2 to 6 and similar dendritic polymers based thereon.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.