Use of multivalent inorganic cations in the electrochemical treatment of nucletic acid containing solutions
US5607832A · kind A · utility
21Cited by
3References
30Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Nov 18, 1994 |
| Grant date | Mar 4, 1997 |
| Priority date | — |
| Expiry date | Nov 18, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process is described for denaturing native double-stranded nucleic acid material into its individual strands in an electrochemical cell. The process is an electrical treatment of the nucleic acid with a voltage applied to the nucleic acid material by an electrode. The process employs a promoter which is an inorganic multivalent cation such as magnesium ions to speed denaturation. The process may be used in the detection of nucleic acid by hybridrising with a labelled probe or in the amplification of DNA by a polymerase chain reaction or ligase chain reaction.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.