Immunological detection method of pyrethroid compound, haptenic compound, intermediate for the haptenic compound, immunogen and antibody
US5608104A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 27, 1994 |
| Grant date | Mar 4, 1997 |
| Priority date | — |
| Expiry date | Sep 27, 2014 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/5308
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Described is a detection method of a pyrethroid compound which comprises providing a first antibody against the pyrethroid compound, said first antibody having been obtained by immunizing a responder with an immunogen which is a first conjugate between a first carrier and a first haptenic compound represented by the following formula (1) or (2): ##STR1## mixing the first antibody with the sample solution, causing any unreacted portion of the first antibody to be bound on a second conjugate as a coated antigen, said second conjugate being formed of a second carrier and a second haptenic compound and having been coated on a surface of a solid phase carrier, whereby an antigen-antibody conjugate is obtained; and PA1 reacting the antigen-antibody conjugate with a second antibody against the first antibody, said second antibody having a labelled enzyme linked therewith. This invention has made it possible to conduct easy, economical and quick analysis of a pyrethroid compound contained in our surroundings such as water or soil, plants or foods.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.