Purification of .alpha.-1 proteinase inhibitor using novel chromatographic separation conditions
US5610285A · kind A · utility
51Cited by
6References
4Claims
0Family size
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Key dates
| Filing date | Aug 24, 1994 |
| Grant date | Mar 11, 1997 |
| Priority date | — |
| Expiry date | Aug 24, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/83
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Cation chromatography with solutions at pH less than 6.0 and low ionic strength can be utilized to purify human .alpha.-1 proteinase inhibitor (.alpha.-1 PI) from biological fluids including plasma and plasma fractions. The cation chromatography takes advantage of the fact that active .alpha.-1 PI does not bind to the cation column under these conditions but other proteins, including denatured .alpha.-1 PI and albumin, do. The effect is that active .alpha.-1 PI flows through the chromatography column leaving the contaminating proteins behind. Recovery of .alpha.-1 PI is high and improvement of purity is dramatic.
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