Capillary electrophoresis method using initialized capillary and polyanion-containing buffer and chemical kit therefor
US5611903A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 22, 1995 |
| Grant date | Mar 18, 1997 |
| Priority date | — |
| Expiry date | Mar 22, 2015 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44747
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A capillary electrophoresis detection and/or analysis method comprising the following steps: PA1 rinsing a ready-to-use capillary with an initiator to create an initialized capillary, PA1 adding a capillary buffer into the initialized capillary, PA1 injecting a sample to be analyzed (possibly diluted with a sample diluent) into the initialized capillary, PA1 optionally, adding a cathodic buffer to the cathodic end of the capillary, and PA1 submitting the sample to capillary electrophoresis. In this method, the capillary and/or cathodic buffer comprise(s) a polyanion or a mixture of polyanions. The polyanion or the mixture of polyanions is included at least in the capillary or the cathodic buffer. The method can additionally comprise rinsing the capillary with NaOH after electrophoresis. The present invention also includes a capillary electrophoresis detection and/or analysis unit, including an initiator and a capillary buffer and/or a cathodic buffer. The capillary and/or the cathodic buffer contain a polyanion or a mixture of polyanions.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.