DNA base sequence containing regions involved in the production and secretion of a protein, recombinant DNA including the whole or a part of the DNA base sequence, and method of producing proteins by use of the recombinant DNA
US5612192A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Dec 20, 1994 |
| Grant date | Mar 18, 1997 |
| Priority date | — |
| Expiry date | Dec 20, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/036
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A neutral protease gene of Bacillus amyloliquefaciens is cloned, which gene comprises the promoter region, the ribosome binding region, the region involved in the secretion of the neutral protease, the region consisting of the structural gene for the neutral protease, and the terminator region. Each of the regions is useful as a material for construction of a recombinant DNA used for the production of proteins by culturing a host bacterium transformed with the recombinant DNA. For example, the extracellular production of neutral protease in a large amount can be accomplished by culturing B. subtilis transformed with a recombinant DNA comprising pUB110 and the neutral protease gene.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.