Retrovirus infecting primate bone marrow cells and harvesting both non-adherent and adherent cells
US5612206A · kind A · utility
11Cited by
0References
13Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 21, 1994 |
| Grant date | Mar 18, 1997 |
| Priority date | — |
| Expiry date | Jun 21, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2810/855
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method is described for preparing primate bone marrow cells containing a DNA sequence of interest the method being first the isolation of bone marrow cells from a primate and then co-culturing the bone marrow cells with retrovirus producer cells. The retrovirus contains the DNA sequence of interest and infects the bone marrow cells during co-culture. To obtain bone marrow cells that contain the DNA sequence of interest, non-adherent bone marrow cells and adherent cells are harvested.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.