Method for the inducible production of proteins in genetically modified eukaryotic host-cells multiplied in vivo
US5614381A · kind A · utility
6Cited by
2References
13Claims
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Key dates
| Filing date | Apr 13, 1995 |
| Grant date | Mar 25, 1997 |
| Priority date | — |
| Expiry date | Apr 13, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2830/85
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
In the production of proteins of biological interest by means of a stress inducible gene expression unit/eukaryotic host cell system, the transformed cell lines are multiplicated by tumour growing in immunodefficient warm-blooded animals, after which the multiplicated cells are cultured in vitro and subjected to stress, whereby expression occurs in high yield. In vivo multiplication rates of 10.sup.5 -10.sup.6 the innoculated quantity/2 weeks are reported without any loss of the latent inducible expression capacity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.