Hepatitis B virus surface proteins with reduced host carbohydrate content
US5614384A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 4, 1995 |
| Grant date | Mar 25, 1997 |
| Priority date | — |
| Expiry date | Jan 4, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2730/10122
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
In order to produce hepatitis B virus (HBV) surface proteins in the form of particles with substantially reduced entrapped carbohydrate content, DNA encoding the HBV surface proteins was expressed in a recombinant yeast host which is deficient in its ability to glycosylate proteins. These HBV surface proteins display the antigenie sites genetically encoded by the S domain of the HBV virion envelope open reading frame and contains substantially reduced levels of entrapped carbohydrate when compared with HBsAg particles produced in "wild-type" yeast cells. These particles are useful as a vaccine for both the active and passive treatment or prevention of disease and/or infection caused by HBV or other agents serologically related to HBV.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.