Method and monoclonal antibodies for vitamin B12 determination

US5614394A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Nicholas Hoyle · Tutzing, DE
• Gunter Pappert · Tutzing, DE
• Michael Grol · Feldafing, DE
• Christa Hubner-Parajsz · Tutzing, DE

Key dates

• Filing date: May 16, 1995
• Grant date: Mar 25, 1997
• Expiry date: May 16, 2015

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S436/815
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method for making an monoclonal antibody specific for vitamin B12 by immunizing mice with vitamin B12-b-acid and isolating hybridomas that produce monoclonal antibodies which have an affinity constant for B12 greater than 5.times.10.sup.9 l/mol is disclosed.