Selection of ribozymes that efficiently cleave target RNA

US5616459A · kind A · utility

Assignee

• The Public Health Research Institute Of the City of New York, Inc. · US

Inventors

• Fred R. Kramer · Yonkers, US
• David Dubnau · New York, US
• Karl Drlica · New York, US
• Abraham Pinter · Brooklyn, US

Key dates

• Filing date: Aug 18, 1992
• Grant date: Apr 1, 1997
• Expiry date: Aug 18, 2012

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2310/121
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Mutant ribozymes are screened by culturing cells whose survival is dependant upon cleavage of RNA by a ribozyme, which cleavage causes the cells to survive in the presence of an agent which otherwise would kill the cells, and selecting cells which survive.