Genomic DNA sequences encoding human BSSL/CEL

US5616483A · kind A · utility

Assignee

• ASTRA CO., LTD. · SE

Inventors

• Karl Gunnar Bjursell · Partille, SE
• Peter CARLSSON · Floda, SE
• Curt S. M. Enerback · Mölndal, SE
• Stig Lennart Hansson · Wasserburg am Inn, DE
• Ulf F. P. Lidberg · Göteborg, SE
• Jeanette Annika Nilsson · Göteborg, SE
• Jan Törnell · Mölndal, SE

Key dates

• Filing date: May 27, 1993
• Grant date: Apr 1, 1997
• Expiry date: May 27, 2013

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2830/008
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present invention relates to a DNA molecule containing intron sequences and encoding a human protein which is, depending on the site of action, called Bile Salt-Stimulated Lipase (BSSL) or Carboxyl Ester Lipase (CEL). The DNA molecule is advantageously used in the production of recombinant human BSSL/CEL, preferably by means of production in transgenic non-human mammals. The recombinant human BSSL/CEL can be used as a constituent of infant formulas used for feeding infants as a substitute for human milk, or in the manufacture of medicaments against e.g. fat malabsorption, cystic fibrosis and chronic pancreatitis.