DNA sequence which binds transcriptional regulatory proteins activated in response to various cytokines and uses thereof
US5616489A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Feb 24, 1995 |
| Grant date | Apr 1, 1997 |
| Priority date | — |
| Expiry date | Feb 24, 2015 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
An oligonucleotide comprising the DNA sequence TTTCCNGGAAA (SEQ ID NO:1) in single stranded or double stranded form, or a derivative thereof, binds to transcriptional regulatory proteins induced by cytokines, growth factors or hormones. Such oligonucleotides are useful in detecting, identifying or purifying transcriptional regulatory proteins. Recombinant DNA constructs comprising the oligonucleotide sequence operably linked to a promoter and a reporter DNA sequence and cells transfected therewith are also disclosed. Also disclosed are DNA-binding proteins other than p91 which are inducible in a cell by a cytokine, growth factor or hormone, and which, when tyrosine phosphorylated, bind to the above oligonucleotide DNA sequence.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.