Enzymatic fluorometric assay for adenylate cyclase
US5618665A · kind A · utility
64Cited by
2References
16Claims
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Key dates
| Filing date | Jan 21, 1994 |
| Grant date | Apr 8, 1997 |
| Priority date | — |
| Expiry date | Jan 21, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/963
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for measuring the amount of adenylate cyclase without the use of radioactive reagents is provided. The method comprises combining a sample of physiological material containing an amount of cAMP with (a) a mixture of enzymes effective to eliminate any other endogenous adenine nucleotides which may be present in the sample; and (b) an amount of alkaline phosphatase effective to eliminate any glucose-6-phosphate present in the sample. The cAMP present in said sample is then converted to AMP and the amount of AMP measured, which may then be correlated to the amount of cAMP and AC present in the sample.
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