Method of measuring the total ketone body and a sample reagent
US5618686A · kind A · utility
12Cited by
9References
6Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 4, 1994 |
| Grant date | Apr 8, 1997 |
| Priority date | — |
| Expiry date | Mar 4, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/11
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of measuring the total ketone body in a sample comprising converting acetoacetic acid in the sample to 3-hydroxybutyric acid in advance and subsequently converting the whole 3-hydroxybutyric acid including 3-hydroxybutyric acid existing in the sample originally to acetoacetic acid to lead the reduction of nicotinamide adenine dinucleotide according to the action of 3-hydroxybutyrate dehydrogenase and a sample reagent thereof is disclosed. According to this invention an assay of the total ketone body in samples can be carried out conveniently, highly precisely and quickly.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.