Determination of the number of fluorescent molecules on calibration beads for flow cytometry
US5620842A · kind A · utility
47Cited by
4References
14Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 29, 1995 |
| Grant date | Apr 15, 1997 |
| Priority date | — |
| Expiry date | Mar 29, 2015 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/101666
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A rapid, simple method for preparing beads for calibrating flow cytometers which contain a known number of fluorophores per bead is presented. Briefly, the invention utilizes beads coated with a stable complex of a fluorophore and an enzyme. the enzymatic activity of a known number of beads gives an accurate measure of fluorophore density on those beads.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.