Molecular recognition at surfaces derivatized with self-assembled monolayers
US5620850A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 26, 1994 |
| Grant date | Apr 15, 1997 |
| Priority date | — |
| Expiry date | Sep 26, 2014 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2610/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An article suitable for use as a biosensor includes a species of a formula X--R--Ch adhered to a surface of the article as part of a self-assembled monolayer. X is a functionality that adheres to the surface, R is a spacer moiety, and Ch is a chelating agent. A metal ion can be coordinated by the chelating agent, and a polyamino acid-tagged biological binding partner of a target biological molecule coordinated to the metal ion. A method of the invention involves bringing the article into contact with a medium containing or suspected of containing the target biological molecule and allowing the biological molecule to biologically bind to the binding partner. The article is useful particularly as a surface plasmon resonance chip.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.