Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same
US5622822A · kind A · utility
18Cited by
6References
18Claims
0Family size
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Key dates
| Filing date | Sep 13, 1994 |
| Grant date | Apr 22, 1997 |
| Priority date | — |
| Expiry date | Sep 13, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6806
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Nucleic acids can be made available for amplification or other treatment after lysis by contacting the lysate with polyethyleneimine to form a precipitate with the nucleic acids. The nucleic acids are then released from the precipitate by contact with a strong base, and the released nucleic acids are kept in solution with an anionic phosphate ester surfactant. This method for preparing specimen samples is simple and quite rapid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.