DNA sequencing by mass spectrometry via exonuclease degradation
US5622824A · kind A · utility
262Cited by
4References
28Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Feb 10, 1995 |
| Grant date | Apr 22, 1997 |
| Priority date | — |
| Expiry date | Feb 10, 2015 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N35/1067
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods for determining the sequence of nucleic acids by cleaving the nueleic acid unilaterally from a first end with an exonuclease activity to sequentially release individual nucleotides, identifying each of the sequentially release nucleotides by mass spectrometry, and determining the sequence of the nueleic acid from the identified nucleotides are disclosed. The method is amenable to multiplexing for simultaneously determining more than one nucleic acid sequence.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.