Bilirubin oxidase from alfalfa and use of the enzyme

US5624811A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Gunter Lang · Wernau, DE
• Ingo Bohn · Birkenau, DE
• Hans-Willi Krell · Penzberg, DE

Key dates

• Filing date: Mar 18, 1994
• Grant date: Apr 29, 1997
• Expiry date: Mar 18, 2014

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/146666
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Enzyme specific for bilirubin which has a phenol oxidase activity of less than 0.5% and an activity for biliverdin of less than 10%, a broad pH optimum as well as a good thermostability. The enzyme is obtainable from plants such as alfalfa and is suitable for the determination as well as for the degradation of bilirubin in particular in biological liquids.