Patent · US Expired

Three-dimensional liver cell and tissue culture system

US5624840A · kind A · utility

70Cited by
28References
10Claims
0Family size

Assignee

Inventors

Key dates

Filing dateMay 31, 1995
Grant dateApr 29, 1997
Priority date
Expiry dateMay 31, 2015

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12N2533/70
  • WIPO fieldPharmaceuticals
  • WIPO sectorChemistry

Abstract

The present invention relates to a three-dimensional cell and tissue culture system. In particular, it relates to this culture system for the long term culture of liver cells and tissues in vitro in an environment that more closely approximates that found in vivo. The culture system described herein provides for proliferation and appropriate liver cell maturation to form structures analogous to tissue counterparts in vivo. The resulting liver tissues survivo for prolonged periods, perform liver-specific functions, and maintain hepatic tissue architecture following in vivo implantation. The liver cultures have a variety of applications ranging from transplantation or implantation in vivo, to screening cytotoxic compounds and pharmaceutical compounds in vitro, to the production of biologically active molecules in "bioreactors" and to the construction of extracorporeal liver assist device.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.