Purification of proteins

US5625041A · kind A · utility

Assignee

• Delta Biotechnology Limited · GB

Inventors

• Richard A. Johnson · Murrysville, US
• Alan Victor Quirk · Loughborough, GB
• John Rodney Woodrow · Nottingham, GB

Key dates

• Filing date: Nov 22, 1994
• Grant date: Apr 29, 1997
• Expiry date: Nov 22, 2014

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K14/765
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Problem: when proteins are purified using a protein-binding dye immobilised on a chromatographic matrix, the dye or a portion/derivative may leak into the eluant. Solution: an ion-exchange resin (e.g. Dowex-1) and a disrupting material (e.g. salt and a fatty acid such as sodium octanoate) are used to separate the dye from the protein.