Synthetic DNA sequence having enhanced insecticidal activity in maize

US5625136A · kind A · utility

Assignee

• Ciba-Geigy · US

Inventors

• Michael G. Koziel · Clive, US
• Nalini M. Desai · Cary, US
• Kelly S. Lewis · Hillsborough, US
• Vance Cary Kramer · Hillsborough, US
• Gregory W. Warren · Cary, US
• Stephen V. Evola · Apex, US
• Lyle D. Crossland · Chapel Hill, US
• Martha S. Wright · Cary, US
• Ellis J. Merlin · Raleigh, US
• Karen L. Launis · Wake Forest, US
• Steven Rothstein · Clive, US
• Cindy G. Bowman · Cary, US
• John L. Dawson · Chapel Hill, US
• Erik Dunder · Chapel Hill, US
• Gary M. Pace · Cary, US
• Janet L. Suttie · Raleigh, US

Key dates

• Filing date: Sep 25, 1992
• Grant date: Apr 29, 1997
• Expiry date: Sep 25, 2012

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02A40/146
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

DNA sequences optimized for expression in plants are disclosed. The DNA sequences preferably encode for an insecticidal polypeptides, particularly insecticidal proteins from Bacillus thuringiensis. Plant promoters, particular tissue-specific and tissue-preferred promoters are also provided. Additionally disclosed are transformation vectors comprising said DNA sequences. The transformation vectors demonstrate high levels of insecticidal activity when transformed into maize.