Monoclonal antibody against ricin A chain

US5626844A · kind A · utility

Assignee

• The United States of America as represented by the Secretary of the Army · US

Inventors

• Paul V. Lemley, Jr. · Warren, US
• D. Craig Wright · Gaithersburg, US

Key dates

• Filing date: Dec 27, 1994
• Grant date: May 6, 1997
• Expiry date: Dec 27, 2014

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61K38/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Monoclonal antibodies against the A chain of ricin have been found to be ective in protecting mammals from morbidity arising from exposure to ricin toxin. The neutralizing action of the antibodies does not appear to be mediated by complement or by immunoprecipitation. The antibodies of the invention are characterized as of isotype IgG1 having the binding characteristics which include: a) binding specifically to the neutralizing epitope of the ricin A chain and b) providing in vitro protection of at least 95% of EL-4 cells from 100 .eta.g/mL ricin challenge when said antibody is present in the tissue culture at a level of at least 1000 .eta.g/mL.