Enhancement of lipid cationic transfections in the presence of serum
US5627159A · kind A · utility
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Key dates
| Filing date | Oct 27, 1994 |
| Grant date | May 6, 1997 |
| Priority date | — |
| Expiry date | Oct 27, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/87
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method is provided for enhancing transfection efficiency of eukaryotic cells comprising contacting said cells with a lipid aggregate comprising nucleic acid and a polycationic lipid composition in the presence of a polycationic compound and serum. The polycationic compound is preferably Polybrene.TM., and the lipid aggregate preferably comprises liposomes of nucleic acid and LipofectAMINE.TM..
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.