Method and detector for separation processes

US5627643A · kind A · utility

Inventors

• Staffan Birnbaum · Lund, SE
• Jonas Johansson · Delta, CA
• Per-Olof Larsson · Helsingborg, SE
• Akiyoshi Miyabayashi · Malmö, SE
• Klaus Mosbach · Halmstad, SE
• Staffan Nilsson · Alvesta, SE
• Sune Svanberg · Lund, SE
• Karl-Gustav Wahlund · Lund, SE

Key dates

• Filing date: Oct 7, 1994
• Grant date: May 6, 1997
• Expiry date: Oct 7, 2014

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N30/6082
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention comprises a method Of detecting and quantifying components separated by capillary electrophoresis or other techniques using thin capillaries in the separation. The method of the present invention comprises irradiating the whole or large parts of the capillary at short time intervals and detecting the fluorescence originating from the sample components, preferably by a CCD detector (charge coupled device). Thereby, an instantaneous image of the progress of the separation process is obtained. The present invention further comprises an apparatus for performing this detection.