Process for disintegrating cell dispersions or cell suspensions by means of ultrasonication for the purpose of isolating cell constituents
US5629185A · kind A · utility
11Cited by
3References
4Claims
0Family size
Inventors
Key dates
| Filing date | Aug 1, 1994 |
| Grant date | May 13, 1997 |
| Priority date | — |
| Expiry date | Aug 1, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12M47/06
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process capable of being continuously carried out, for disintegrating cell material in the form of dispersions or suspensions in water for the purpose of obtaining cell constituent. Selected parameters permit avoidance of use of solid ultrasonication activators and the establishment of a particular geometry form for the acoustic irradiation container. The parameters include sonotrode immersion angle, length of immersion, ratio of extent of immersion of the sonotrode relative to the acoustic irradiation volume and the ratio of extent of immersion to the solid matter content of the medium to be sonicated.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.