Application of novel DNA fragments as a coding sequence for a signal peptide for the secretion of mature proteins by recombinant yeast, expression cassettes, transformed yeast and corresponding process for the preparation of proteins

US5631144A · kind A · utility

Assignee

• TRANSGENE SA · FR

Inventors

• Yves Lemoine · Strasbourg, FR
• Martine Nguyen · Bischwiller, FR
• Tilman Achstetter · Oberkirch, DE
• Jean-Marc Reichhart · Strasbourg, FR

Key dates

• Filing date: Feb 7, 1995
• Grant date: May 20, 1997
• Expiry date: Feb 7, 2015

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/61
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to new DNA fragments and to their application as a DNA coding fragment for a signal peptide which can be used for the secretion of proteins, said peptide including a sequence of amino-acids which show a degree of correspondence of at least 60% with the sequence of amino-acids (I) or (II), preferably with the sequence (II). The sequences (I) and (II) are as follows: (I) Arg-Phe-Ser-Thr-Thr-Leu-Ala-Thr-Ala-Ala-Thr-Ala-Leu-Phe-Phe-Thr-Ala-Ser-Gl n. (II) Arg-Phe-Ser-Thr-Thr-Leu-Ala-Thr-Ala-Ala-Thr-Ala-Leu-Phe-Phe-Thr-Ala-Ser-Gl n-Val-Ser-Ala.