Ligation with hammerhead ribozymes
US5633133A · kind A · utility
48Cited by
0References
15Claims
0Family size
Inventors
Key dates
| Filing date | Jul 14, 1994 |
| Grant date | May 27, 1997 |
| Priority date | — |
| Expiry date | Jul 14, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/315
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The claimed invention is drawn to a method for ligation of an enzymatic nucleic acid molecule having a hammerhead motif to a separate nucleic acid molecule. The method includes use of ligation enhancing conditions in which ligation is enhanced at least two fold over that observed in a medium containing only Mg.sup.2+ as a divalent cation at 20.degree. C.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.