Reaction vessel for immunological analysis of aerosols

US5633140A · kind A · utility

Assignee

• DRAGERWERK AKTIEGESELLSCHAFT · DE

Inventors

• Kerstin Wex · Bocholt, DE
• Thomas Wuske · Malente, DE
• Andreas Manns · Lübeck, DE

Key dates

• Filing date: Dec 22, 1994
• Grant date: May 27, 1997
• Expiry date: Dec 22, 2014

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/25875
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A unitary collection and displacement immunoassay device for determining a toxic substance adhered to aerosols in the air is provided. The device comprises an elongated body having proximal and distal ends and encloses a space defining a collection chamber, a reaction chamber and a measurement chamber, each chamber separated by a liquid flow barrier which delays liquid transport between chambers until a vacuum source is applied to the distal end of the test device to pull liquid into the next chamber. The collection chamber contains a solution for solubilizing the toxic substance from the aerosols and a semi-permeable means for separating the solubilized toxic substance from the aerosols. The reaction chamber contains a labelled immunoreactant bound to a solid phase carrier material. The labelled reagent can be displaced by the solubilized toxic substance. The measurement chamber contains a color generating system which reacts with displaced labelled reagent to produce a colored product in proportion to the concentration of the toxic substance.