Method for the quantitative determination of D-3-hydroxybutyric acid and acetoacetic acid, and analytical reagent therefor
US5633143A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 26, 1994 |
| Grant date | May 27, 1997 |
| Priority date | — |
| Expiry date | May 26, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/104998
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is a method for the quantitative determination of D-3-hydroxybutyric acid and acetoacetic acid, which comprises reacting a biological sample containing D-3-hydroxybutyric acid and acetoacetic acid, with a reagent comprising: (1) a D-3-hydroxybutyrate dehydrogenase, (2) A.sub.1 and (3) B.sub.1, the components (1), (2) and (3) participating in the following cycling reaction: ##STR1## thereby effecting the enzymatic cycling reaction, and measuring a change in the amount of A.sub.2 formed or the amount of B.sub.1 consumed. Also disclosed is an analytical reagent comprising the components (1), (2) and (3) for use in the above method. The method and the analytical reagent ensure rapidness and accuracy in the determination of D-3-hydroxybutyric acid and acetoacetic acid, even with the use of a small quantity of a biological sample, so that they are very useful in application fields, such as clinical diagnosis and food testing.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.