Patent · US Expired

Method for culturing Chinese hamster ovary cells

US5633162A · kind A · utility

183Cited by
3References
18Claims
0Family size

Assignee

Inventors

Key dates

Filing dateMar 4, 1994
Grant dateMay 27, 1997
Priority date
Expiry dateMar 4, 2014

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12N2501/395
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

A biochemically defined culture medium for culturing engineered Chinese hamster ovary (CHO) cell lines, which is essentially free from protein, lipid and carbohydrate isolated from an animal source, having water, an osmolality regulator, a buffer, an energy source, amino acids including L-glutamine, an inorganic or recombinant iron source, and a synthetic or recombinant growth factor, and optionally non-ferrous metal ions vitamins and cofactors. Also cells adapted to grow in such a culture medium.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.