Rapid assays for amplification products
US5635347A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 28, 1994 |
| Grant date | Jun 3, 1997 |
| Priority date | — |
| Expiry date | Jan 28, 2014 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2458/30
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of detecting a nucleic acid sequence of interest in the amplification product of a polymerase chain reaction or other primer directed reaction comprising the steps of: PA1 (a) incorporating in a polymerase chain reaction mixture or other primer directed reaction mixture at least one nucleic acid sequence complementary to said nucleic acid sequence of interest labeled (i) at the 3' end thereof, or (ii) at the 3' and the 5' end thereof with a compound capable of electrochemiluminescence; PA1 (b) conducting a polymerase chain reaction or other primer directed reaction; and PA1 (c) measuring the electrochemiluminescence of labeled amplification product.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.