Reagents and kits for determining polymerase activity

US5635350A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Josef Eberle · Freising, DE
• Rudolf Seibl · Penzberg, DE
• Christoph Kessler · Munich, DE
• Bernhard König · Bobingen, DE

Key dates

• Filing date: Feb 3, 1995
• Grant date: Jun 3, 1997
• Expiry date: Feb 3, 2015

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/81
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Method of determining polymerase activity by incubating the polymerase with a template nucleic acid, one detectable mononucleoside triphosphate and one immobilizable nucleoside triphosphate, binding the immobilizable nucleotides to a solid phase and detecting the bound delectable nucleotides and tests based thereon.