Method for the introduction of genes into mammalian cells by a soluble molecular complex comprising a receptor ligand and a polycation
US5635383A · kind A · utility
61Cited by
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2Claims
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Key dates
| Filing date | Jan 4, 1995 |
| Grant date | Jun 3, 1997 |
| Priority date | — |
| Expiry date | Jan 4, 2015 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K47/62
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A targetable gene delivery system is provided for introducing foreign genes into mammalian cells. The system employs a soluble targetable DNA complex and utilizes receptor-mediated endocytosis to endow cell specificity. The soluble DNA-carrying complex is formed by non-covalently binding a ligand conjugate with the foreign gene. The conjugate, in turn, is formed by bonding receptor-specific ligands such as asialoglycoproteins to polycations such as polylysine through covalent bonds such as disulfide bonds. The system exhibits a high degree of cell specificity and offers potential for the treatment of inherited genetic disorders.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.