Isolated DNA encoding a nitrilase polypeptide, hosts containing, and expression thereof optionally assisted by a E. coli GroE chaperone polypeptide
US5635391A · kind A · utility
28Cited by
1References
18Claims
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Key dates
| Filing date | Jun 5, 1995 |
| Grant date | Jun 3, 1997 |
| Priority date | — |
| Expiry date | Jun 5, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/78
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to novel polypeptides having a nitrilase activity and to the genetic tools for producing them, namely: PA1 the DNA sequence coding for a polypeptide having a nitrilase activity and capable of hydrolyzing nitriles to carboxylates, PA1 an analog of this sequence resulting from the degeneracy of the genetic code, PA1 a DNA sequence hybridizing with one of these sequences or a fragment thereof and coding for a polypeptide having a nitrilase activity, PA1 expression cassettes and microorganisms enabling them to be obtained. Application: enzymatic conversion of nitriles to carboxylates.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.